Reduction of canine plasminogen leads to an expanded molecule which precipitates.

Reduction of canine plasminogen leads to an expanded molecule which precipitates.

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Canine plasminogen is made up of seven domains.In each domain there are several cysteines that are Furniture linked by disulfide bonds.Reduction of a limited number of the cystines destabilizes the protein such that it precipitates.The bond or bonds that are broken provide Web Incense about 14 kcal of stabilization energy.Circular dichroism and dynamic light scattering indicate that there is probably an intermediate that is formed prior to precipitation and that the intermediate is somewhat larger than the compact form of plasminogen.